Daybook Analysis - Version 1.10.0 - October 2020
This analysis only concerns microscopes equipped with a motorized XY stage.
Tiles imaging is typically carried out to ensure adequate resolution when imaging a large specimen.
When acquiring tiles images, the XY stage may not reposition to exactly the same location in a repeatable manner, distorting the stitched image of the specimen. It is therefore important to ensure that the repositioning step is significantly repeatable, so that it does not prevent the accurate tiling of the images.
Sources of stage repositioning non-repeatability are typically changes to environmental conditions (such as temperature fluctuations and air flow) or stage malfunctions.
The “stage repositioning repeatability” analysis measures the lateral drift magnitude from the motorized XY stage after many round-trips, to ensure it is within the specifications of the manufacturer or experimentalist.
The “stage repositioning repeatability” analysis is associated with any “repositioning cross” pattern (Pattern family H - see Figure 1).
Figure 1: Example image of a “repositioning cross” pattern, fulfilling the acquisition recommendations.