Daybook Analysis - Version 2.0.0 - October 2024
This analysis only concerns microscopes equipped with a motorized XY stage.
Timelapse imaging is often carried out to track the motion of a specimen.
When acquiring timelapse images, the XY stage may drift, contributing to the specimen motion. It is therefore important to measure the stage drift, so that it can be corrected for, and does not introduce bias into the measurement of the specimen trajectory. This can also trigger a maintenance session.
Sources of stage drift over time typically come from environmental conditions (such as temperature fluctuations and air flow) or stage malfunctions.
The “stage drift during timelapse” analysis measures the lateral drift magnitude from the motorized XY stage during timelapse imaging, to ensure it is within the specifications of the manufacturer or experimentalist.
The “stage drift during timelapse” analysis is associated with any “repositioning cross” pattern (Pattern family H - see Figure 1).
Figure 1: Example image of a “repositioning cross” pattern, fulfilling the acquisition recommendations.