Daybook Analysis - Version 2.0.0 - October 2024
This analysis only concerns microscopes that can acquire fluorescence emission spectra.
Although conceived primarily as imaging systems, fluorescence microscopes can also acquire emission spectra, with a better spatial resolution but poorer spectral resolution than conventional spectrometers. The spectral response of a microscope expresses the responsivity of the imaging system versus the emission wavelengths.
Correctly interpreting the emission spectra measured with a microscope requires knowledge of its spectral response.
The “spectral response” analysis provides the normalized detection efficiency versus the emission wavelengths, as well as quantitative parameters such as the spectral roll-off.
The “spectral response” analysis is associated with the “target” (Pattern family A), “word ARGOLIGHT” (Pattern family J), or “repositioning cross” (Pattern family H) patterns (see Figure 1).
Figure 1: Example images of the “target”, “word ARGOLIGHT” and “repositioning cross” patterns.