Quick Start Lateral resolution

Ve. 1225 1

1. Prep the workspace

Gather the kit: get the slide out of the storage box, have the latest version of Daybook 4 software installed on the workstation beforehand (internet connection required). Have your Daybook 4 account created, and license entered (the license code ships with the slide). https://argolight.com/products/daybook-4/
Clean the glass surface and your objective with a lens tissue lightly soaked with a cleaning solvent (ethanol, isopropyl alcohol...)
Power on the microscope and warm up the light source for at least one hour (especially for confocal).

Select a low‑magnification objective*(5x recommended)* first so you can locate the patterns quickly without hunting with a high‑NA lens. Use the DAPI channel (350‑405 nm). The pattern shines brighter in DAPI.
Place the Argolight slide on the stage, face‑up (for upright microscopes).
Turn on the camera. Select the auto-exposure option in your acquisition software.
•Coarse‑align the slide center with the camera’s field of view using the XY and Z translation knobs.
Select the pattern of interest for your quality control (the Gradually Spaced Lines for Lateral resolution analysis). Go to the center of the pattern of interest.

Tips: The line spacings run from 0 nm to ~390 nm, perfect for probing the smallest resolvable feature of the imaging system.
Then, navigate Z. Adjust the focus