Daybook Analysis - Version 2.0.0 - October 2024
The optical power measured at the sample plane is a key parameter in microscopy setups to troubleshoot any issue or perform quantitative microscopy.
Measuring the optical power at regular intervals using the same illumination settings enables troubleshooting: for example, change in the power of the light sources, drop in the transmission of filters, or laser-to-fiber coupling misalignment.
Measuring the optical power before and after an imaging campaign shows whether the light source power has fluctuated during the campaign, and so whether it has affected the intensity values in the images.
Measuring the optical power before an imaging campaign using the same illumination settings as for a previous imaging campaign ensures that the same optical power is applied to the sample so that the illumination conditions are reproduced.
Measuring the optical power allows this value to be reported in scientific publications, so that other researchers can reproduce the same illumination conditions. This will help to combat difficulties in replication of experimental conditions.
The “power meter” interface allows the measurement of live optical power, live irradiance, time-lapse, and power vs setting.