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Daybook Analysis - Version 2.0.0 - October 2024

The line spread function (LSF) determines the spreading behavior of light by a line object. Fitting the LSF with a mathematical function (double Gaussian, double Lorentzian, double Sech²) allows the calculation of the full width at half maximum (FWHM), which is a parameter related to the lateral resolution of the imaging system.

The “line spread function” analysis provides information, in the XY plane, on how light spreads from a line, as well as quantitative parameters such as the FWHM of the LSF and the signal-to-noise ratio (SNR) value in the image.

I. IMAGE ACQUISITION PROCEDURE

The “ring spread function” analysis is associated with the “field of rings” pattern (Pattern family B - see Figure 1), or any single ring within any pattern.

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Figure 1: Example image of the “field of rings” pattern, with a cross at the center, fulfilling the acquisition recommendations.

1. ACQUISITION RECOMMENDATIONS

2. IMAGING THE PATTERN

II. IMAGE ANALYSIS PROCEDURE

1. LAUNCHING THE ANALYSIS

2. ANALYSIS SETTINGS