Daybook Analysis - Version 1.10.0 - October 2023
Field uniformity is a well-known parameter related to the inhomogeneous spatial distribution of the intensity in the image of an even object.
For any fluorescence microscope, knowledge of the field uniformity is important when measuring features intensity. For intensity quantification in images of biological samples, the field uniformity shall be known, and eventually corrected, to have access to accurate measurements.
The “field uniformity” analysis provides the spatial distribution of the intensity, due to illumination and detection inhomogeneities, within the field of view.
The “field uniformity” analysis is associated with the “field of rings” pattern (Pattern family B - see Figure 1).
Figure 1: Example image of the “field of rings” pattern, with a cross at the center, fulfilling the acquisition recommendations.