Daybook Analysis - Version 2.0.0 - October 2024
Field distortion is an optical aberration inducing a spatial deformation of the imaged object, usually at the corners of the image. In the presence of field distortion, the magnification is not constant over the field of view; it is dependent on the XY coordinates.
For any fluorescence microscope, knowledge of the field distortion is important when spatial information in an image is being measured. For quantification of positions, lengths, areas or volumes in images of biological samples, the field distortion should be known, and eventually corrected for, to ensure accurate measurements.
The “field distortion” analysis provides the lateral shifts, due to distortion, within the field of view.
The “field distortion” analysis is associated with the “field of rings” pattern (Pattern family B - see Figure 1).
Figure 1: Example image of the “field of rings” pattern, with a cross at the center, fulfilling the acquisition recommendations.