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The Argo-WP (Well Plate) is specifically designed to assess and monitor the performance of high-content screening (HCS) and high-throughput screening (HTS) fluorescence imaging systems that can image each well of 96-well cell culture plates with high resolution (to look at cell populations for instance). The Argo-WP is compatible with system magnifications ranging from 10 up to 60×. It contains the third generation of Argoglass®.

The Argo-WP is not compatible with imaging modalities based on total internal reflection (TIRF), fluorophore localization (PALM, STORM, DNA-PAINT), and stimulated emission by depletion (STED).

This product is composed of:

• An aluminum plate that includes four pieces of Argoglass® with embedded fluorescent patterns inside.
• A dedicated software, Daybook, to generate, track and export quality control data.

The pieces of Argoglass® consist of a special glass substrate with different fluorescent patterns embedded inside. The Argo-WP is designed to quality-control many aspects of an HCS or HTS fluorescence imaging system, such as field uniformity, field distortion, lateral co-registration accuracy, intensity response, stage drift during Z-stacking, etc.

The Daybook software has two modules:

• The “Analysis” module, named “Daybook Analysis”: it allows to analyze and extract data (maps, graphics, and metrics) from images of the patterns, in order to measure significant metrics of HCS or HTS fluorescence imaging systems.
• The “Data Manager” module, named “Daybook Data Manager”: it allows to visualize the data generated by the “Analysis” module, monitor the results, and manage the quality control reports.

The best of Argolight technology, adapted into a 96-well plate format, combined with the Daybook software, opens the path to easy, yet reliable and complete, quality control of HCS or HTS fluorescence imaging systems.

I. First use

1. Parcel verification

2. Quick start procedure

II. General handling and care

1. Handling and storage

2. Cleaning