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User guide v.3.1 - December 2015

Argolight’s multidimensional slides are specifically designed for calibrating and monitoring the performances of fluorescence microscope systems (wide-field, confocal, multiphoton). They can be used as a human vision- or a software-assisted calibration tool. The slides consist in a special glass substrate produced at the Argolight facility to insure homogeneity and purity. The glass is set on a stainless steel carrier, featuring the same dimensions as a standard microscope slide (75 mm x 25 mm), except for the thickness which is 1.5 mm, in order to satisfy the required rigidity for this kind of tool.

Different fluorescent patterns are embedded in the glass, at a depth of (180 ± 5) µm from the top surface. This depth was calculated to emulate the presence of a microscope cover-slip (with a thickness of 170 µm and a refractive index of 1.5255 at 546.1 nm), within the paraxial approximation (NA≤0.7). Each fluorescent pattern is intended and designed to respond to a particular calibration issue.

The slides are manufactured at the highest level of precision to insure meaningful test results.

<aside> ♻️ A word about waste management

Argolight’s policy is to offer robust products that last. But if our products become useless to you, please contact us so we can pick them up and recycle them. Please do not throw away the slide with common waste. The composition of the glass requires specific recycling. Thank you.

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I. First use

Inside the package, you will find:

Additional information, such as spectrum data files, is available online at: www.argolight.com/skd

Before starting, check that all these items are present and control if the slide has visible damages. If any damage is observed, please contact Argolight within three days after delivery.

Starting Procedure